Figure 2.

Effect of MCCP treatment on Aβ transgenic expression and Aβ accumulation. (A) Quantitative real-time PCR measured Aβ mRNA levels of CL4176 transgenic worms at 36 h after temperature shifted to 25 °C, average of three biologically independent experiments (ns meant that the difference was not significant). (B) Statistics on the number of Aβ deposits in different groups of worms, and each group had 10 worms, error bar represented the mean ± SEM (* p < 0.05). (C–E) Thioflavin T staining of transgenic C. elegans. unc-54/Aβ_1–42 transgenic C. elegans (CL4176) stained with thioflavin T. Note: muscle thioflavin T-relative deposits. Arrows indicate the Aβ aggregation deposits.