Figure 3.

MCCP exposure upregulated the sir-2.1 transcript level and promoted the DAF-16 nuclear translocation. (A) CL4176 worms were incubated at 16 °C for 48 h on agar media dishes containing MCCP, either moved to control dishes or maintained on MCCP dishes, and then induced to express Aβ_1–42. It was indicated that obvious protection against paralysis was observed even in worms shifted to control dishes before induction of Aβ_1–42 expression. Mean ± SD for control, pretreatment with MCCP and MCCP were 38.00 ± 2.73, 40.10 ± 2.45 and 43.43 ± 3.67 respectively (control with pretreatment with MCCP and MCCP, p < 0.001). (B) The transcript levels of sir-2.1, hsf-1, daf-16, and skn-1 in CL4176 worms treated with and without MCCP were quantified using qRT-PCR. The data was obtained from three independent experiments. (C,D) MCCP treatment was able to accelerate nuclear localization of DAF-16::GFP in C. elegans (TJ356). Data was obtained from three independent experiments (10 worms each). (E,H) The expression of SOD-3 in SOD-3::GFP worms (CF1553 and CF1588) treated with or without MCCP. Data was obtained from three independent experiments (10 worms each). (I,J) The fluorescence intensity from SOD-3::GFP in day-4-adults was calculated by Image pro plus. Comparisons between treatments and controls were significant, * p < 0.05. Data were obtained from three independent experiments (10 worms in each group). (ns meant that the difference was not significant).