Table 1.
Primers used to verify gene expression level by quantitative real-time polymerase chain reaction (qRT-PCR).
| Gene | Primer | Sequence (5′→3′) |
|---|---|---|
| 16S rRNA | 16S rRNA-F | TATCCTTGTTTGCCAGCGAG |
| 16S rRNA-R | CTACGACGCACTTTTTGGGA | |
| tdh | tdh-F | GGCATTTGGATGACCGAAGTA |
| tdh-R | CTGACCAATCGCAACCACTTC | |
| VPA0318 | VPA0318-F | AGGTTACTTAGCGGGTGCG |
| VPA0318-R | TTCACGGTCTTTGATGCC | |
| fliI | fliI-F | TGCGGAACCCATCAACCC |
| fliI-R | CGTCCGTCTTCGCCCAAA | |
| VP1057 | VP1057-F | CGGTTCAATCAGCCCATAC |
| VP1057-R | AACGCTTCTGCGATACCTG | |
| VPA0243 | VPA0243-F | AACGCTTCTGCGATACCTG |
| VPA0243-R | TTGCCATAGTGCGTCGTAGTCG | |
| VPA0202 | VPA0202-F | CGAAGAAGTGATGGTGGTG |
| VPA0202-R | CTCGCATTGGTGAGTTGACG |