Figure 1.

Blue native-PAGE and Western blot analysis of purified human 4F2hc-LAT2 heterodimeric complex. Panel (A) displays a Coomassie Brilliant Blue stained 4-16% gradient blue-native gel of purified 4F2hc-LAT2. Western blot analyses using anti-His (B) and anti-Strep antibodies (C) indicate purity and correct assembly of the complex. The heterodimeric complex migrates to about 230 kDa. The faint band above the 440 kDa marker is in good agreement with a dimer of the heterodimeric complex, possibly due to unspecific aggregation. Note that neither monomeric 4F2hc nor monomeric LAT2 are present in the sample. Five micrograms of purified protein was loaded per lane.