Table 3.
Primer information of selected miRNAs and mRNAs for qRT-PCR validation.
| Gene Name | Primer Sequence | Amplicon (bp) | GenBank/miRBase Accession Number |
|---|---|---|---|
| PPIA | F: CACAAACGGTTCCCAGTTTT | 171 | NM_214353 |
| R: TGTCCACAGTCAGCAATGGT | |||
| ACADS | F: CCAGGGCATCCAGTTCAAGT | 102 | NM_213898 |
| R: TTGCCGGCTCCTTGATGAAT | |||
| CDKN1B | F: TGGAGGGCAAATACGAGTGG | 150 | NM_214316 |
| R: CAATTAAAGGCACCGCCTGG | |||
| ORAI1 | F: TGCATCTGTTTGCGCTGATG | 168 | NM_001173519 |
| R: CCAGGAAGAGCAGTGTACCG | |||
| PRKD2 | F: GGAAAACGTGTTGTTGGCGT | 157 | XM_021094608 |
| R: GTTGTAGCCCTGGTTGAGCA | |||
| PTPN1 | F: TACACCGTCCGACAGCTAGA | 149 | DQ239903 |
| R: CCCGACTCACGGACTTTGAA | |||
| SC5D | F: CGGCTGGTTTCGACTCCTT | 175 | AY609684.1 |
| R: AGCCATCCAGAGGGTGAAAAG | |||
| U6 | F: TTATGGGTCCTAGCCTGAC | - | EU520423 |
| R: CACTATTGCGGGTCTGC | |||
| ssc-miR-212 | ACCTTGGCTCTAGACTGCTTACT | - | MI0022140 |
| ssc-miR-186 | CAAAGAATTCTCCTTTTGGGCTT | - | MI0002456 |
| ssc-miR-128 | TCACAGTGAACCGGTCTCTTT | - | MIMAT0002157 |
| ssc-miR-126 | TCGTACCGTGAGTAATAATGCG | - | MI0016619 |
| unconservative-X-272462 | TGAACGGTGCCTGTGTGGCTAGA | - | / |
| conservative-15-100875 | TCTCTGCTGCGCTCTTTCCTGA | - | / |