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. 2019 Feb 25;20(4):989. doi: 10.3390/ijms20040989

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Evaluation of axonal loss showed a decreased number of phosphorylated neurofilaments (pNF) positive small (1–4<μm) diameter axons within the thoracic and cervical spinal cord at 147 dpi and within the cervical segment at 196 dpi (A). Large (≥4 μm) axons were significantly decreased within the thoracic segment at 98 dpi (B). No significant differences between TMEV-infected groups comparing 147 and 196 dpi were detected. Graphs display box and whisker plots. Significant differences between groups detected by Mann–Whitney U-test were indicated by asterisks (* p ≤ 0.05).