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. 2019 Feb 14;20(4):810. doi: 10.3390/ijms20040810

Figure 3.

Figure 3

Cytotoxicity of HSV-TK expression with Tet-inducible lentiviral vectors. (A–C) Schematic representations of the integrated proviral forms of the Tet-inducible lentiviral vectors, which carried (A) the GFP gene, (B) the HSV-TK-1 gene, and (C) the del-HSV-TK-1 gene. del-HSV-TK-1, truncated HSV-TK gene without the cryptic promoter; TRE, Tet-responsive promoter; rtTA; reverse Tet-controlled transactivator protein gene. 253G1 iPSCs, 1210B2 iPSCs, and HeLa cells transduced with the indicated Tet-inducible lentiviral vectors were cultured in the presence of various concentrations of GCV, with or without 1 μg/mL doxycycline (Dox) for 3–4 days. Cell viability was assessed by the CCK-8 assay. The percent cell viability was calculated relative to cells in the absence of GCV without Dox. There was no significant difference in the results obtained on days 3 and 4 of culture. Data represent the mean ± SEM (n = 4–6). *, p < 0.05; **, p < 0.01.