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. 2019 Feb 15;20(4):853. doi: 10.3390/ijms20040853

Figure 2.

Figure 2

Activin B enhanced the proliferation of hair matrix cells in vivo and in vitro. (A) Representative images of EdU staining (red fluorescence) of vibrissae follicles treated with PBS or 10 ng/mL Activin B at 10 days after treatment. Nuclei were counterstained with Hoechst 33342 (blue fluorescence). (B) Ten vibrissae follicles per group were collected from five mice in each group and the number of EdU-positive cells in the two groups was counted. (C) Human hair germinal matrix cells (HHGMCs) were treated with Activin B at the concentration of 5, 10, 20, 40, 80, or 160 ng/mL. The CCK-8 assay was performed at 12, 24, 48, 72, and 96 h after treatment. (D) HHGMCs treated with 10 ng/mL Activin B or PBS were subjected to EdU assay (green fluorescence). Nuclei were counterstained with Hoechst 33342 (blue fluorescence). (E) The number of positive cells was counted manually in ten fields-of-view randomly selected from each well. The percentages of positive cells were calculated. * p < 0.05; ** p < 0.01, compared with the PBS group. Three independent experiments were conducted per data point. All error bars indicate SEM.