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. 2019 Feb 16;20(4):859. doi: 10.3390/ijms20040859

Figure 2.

Figure 2

HE treatment at 60 mg/kg resulted in significant hippocampal neuroprotection after pilocarpine-induced status epilepticus. (A) Representative photomicrographs showing immunohistochemistry to neuronal nuclei (NeuN) in sham, vehicle (Veh)-, 60 mg/kg of HE- (HE60), 120 mg/kg of HE- (HE120), and 300 mg/kg of HE-treated groups (HE300). Scale bar: 500 μm. (B) A drawing of the hippocampus indicating the region for the quantitative analysis of neuronal survival (shown in red). (C) A graph showing the number of NeuN-expressing cells in the pyramidal cell layer. Note that Veh-treated group showed a marked reduction in the number of NeuN-positive neurons compared to sham at 7 d after status epilepticus (SE). However, 60 mg/kg and 120 mg/kg of HE administration significantly prevented neuronal death against SE, whereas 300 mg/kg of HE showed the similar number of surviving pyramidal neurons compared to Veh-treated animals. Data are shown as mean ± SEM. n = 6 (sham), n = 9 (Veh), n = 6 (HE60), n = 9 (HE120), n = 6 (HE300). * p < 0.05; one-way ANOVA with Tukey’s post-hoc test.