Fig. 4.

Cell-cell interactions between PCNSL cells and macrophages. Lymphoma cells labeled with CFSE were co-cultured with macrophages for 2 days (A), fluorescence signals were evaluated by flow cytometry (B), and then mean fluorescence intensities were compared (C). Lymphoma cells were co-cultured with CM from macrophages and then treated with MTX and radiation (30 Gy) (D). Cell numbers were evaluated with the WST assay following treatment with MTX at various concentrations for 2 days (E). Cells were treated with radiation with or without CM, and cell numbers were assessed with the WST assay following culture for 2 days (F).