Table 1. Comparison of qNMR using 600 MHz 1H NMR and HPLC for determination of concentration of free amino acids (mg/L) of chicken breast meat.
| Metabolite | ppm (δ) | Multiplicity1) | Chicken breast meat (mg/L) | SEM | ||
|---|---|---|---|---|---|---|
| NMR (zg30) | NMR (neosypr1d) | HPLC | ||||
| Leucine | 0.948 | t, CH | 237.77a | 239.53a | 231.84b | 1.599 |
| RSD | 0.25 | 1.96 | 0.62 | |||
| Isoleucine | 0.997 | d, CH3 | 130.88a | 130.03a | 126.74b | 1.205 |
| RSD | 1.24 | 2.48 | 1.48 | |||
| Valine | 0.980 | d, CH3 | 182.85 | 183.17 | 181.99 | 0.773 |
| RSD | 1.06 | 1.94 | 0.86 | |||
| Alanine | 1.460 | d, CH3 | 413.54 | 417.63 | 417.87 | 2.526 |
| RSD | 0.87 | 0.86 | 1.18 | |||
| Glutamic acid | 2.341 | m, CH2 | 440.78 | 438.92 | 439.13 | 3.411 |
| RSD | 1.30 | 1.63 | 0.95 | |||
| Aspartic acid | 2.670 | m, NH | 258.03 | 268.81 | 254.56 | 4.668 |
| RSD | 2.13 | 4.74 | 0.84 | |||
| Glycine | 3.540 | s, CH2 | 230.04 | 227.50 | 228.29 | 2.010 |
| RSD | 1.74 | 1.10 | 1.45 | |||
| Tyrosine | 6.877 | m, CH | 195.09a | 148.86b | 197.10a | 1.568 |
| RSD | 1.00 | 2.36 | 1.10 | |||
| Phenylalanine | 7.320 | d, CH | 124.25 | 128.83 | 122.99 | 1.662 |
| RSD | 2.24 | 3.01 | 2.58 | |||
Standard error of the means (n=9).
1)
Represent peak splitting: s, singlet; d, doublet; t, triplet; dd, doublet of doublet; m, multiplet.
a,b
Mean with different letters within the same row differ significantly (p<0.05).
NMR, nuclear magnetic resonance ; qNMR, quantitative NMR; HPLC, high-performance liquid chromatography; RSD, relative standard deviation.