Table 2. Application of qNMR using 600 MHz 1H NMR with zg30 pulse program (without water suppression) for quantification of free amino acids (mg/L) in beef and pork.
| Beef (Semitendinosus) | RSD | Pork (Longissimus thoracis) | RSD | |
|---|---|---|---|---|
| Leucine | 71.28±0.77 | 1.08 | 85.08±0.85 | 1.00 |
| Isoleucine | 21.40±0.90 | 4.19 | 31.95±1.25 | 3.92 |
| Valine | 60.16±1.83 | 3.04 | 56.59±0.44 | 0.77 |
| Alanine | 219.64±4.01 | 1.83 | 133.79±2.41 | 1.80 |
| Glutamic acid | 122.87±4.40 | 3.58 | 131.92±5.28 | 4.00 |
| Aspartic acid | 88.60±3.54 | 3.99 | 74.93±2.43 | 3.25 |
| Glycine | 128.22±3.71 | 2.89 | 84.81±3.61 | 4.25 |
| Tyrosine | 33.14±1.74 | 5.23 | 33.16±1.13 | 3.40 |
| Phenylalanine | 43.18±2.39 | 5.54 | 47.83±1.99 | 4.15 |
NMR, nuclear magnetic resonance; qNMR, quantitative NMR; RSD, relative standard deviation.