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. 2019 Feb 13;20(4):808. doi: 10.3390/ijms20040808

Table 1.

The phenotype and the main biotechnological parameters of the S. glabra lines (dark conditions) and rooted plants.

Cell Line Phenotype Fresh Biomass
(g L−1)
Dry Biomass
(g L−1)
Stepharine Content (%DW) Stepharine Production
(mg L−1)
S1
Liquid Homogenous
Undifferentiated
cell aggregates
161.6 ± 24.2 11.8 ± 1.4 - -
S2
Liquid Heterogeneous
Somatic embryos 453.3 ± 31 16.8 ± 0.6 0.88 ± 0.06 147.0 ± 12.6
Nodules with roots 1.04 ± 0.03 175.3 ± 5.7
S3
Solid Heterogeneous
Somatic embryos 78.75 ± 8.06 4.6 ± 0.49 0.33 ± 0.01 15.3 ± 3.2
Nodules with roots 0.80 ± 0.19 36.9 ± 8.7
S4
Solid Heterogeneous
Plantlet leafs 119.8 ± 8.3 6.7 ± 0.4 - -
Petiole 0.23 ± 0.04 115.4 ± 2.7
Main stems 0.29 ± 0.09 19.1 ± 6.4
Plantlets roots 0.66 ± 0.06 44.2 ± 4.0
S5
Solid Heterogeneous
Deformed Stems 102 ± 15.2 7.7 ± 0.76 0.20 ± 0.01 15.7 ± 0.7

Suspension culture S1 was cultivated for 10 days, S2 for 25 days and the callus culture S3, S4, S5 for 30 days. The data (mean ± SE) was obtained from six independent experiments with 10 replicates each.