Table 1.
Cell Line | Phenotype | Fresh Biomass (g L−1) |
Dry Biomass (g L−1) |
Stepharine Content (%DW) | Stepharine Production (mg L−1) |
---|---|---|---|---|---|
S1 Liquid Homogenous |
Undifferentiated cell aggregates |
161.6 ± 24.2 | 11.8 ± 1.4 | - | - |
S2 Liquid Heterogeneous |
Somatic embryos | 453.3 ± 31 | 16.8 ± 0.6 | 0.88 ± 0.06 | 147.0 ± 12.6 |
Nodules with roots | 1.04 ± 0.03 | 175.3 ± 5.7 | |||
S3 Solid Heterogeneous |
Somatic embryos | 78.75 ± 8.06 | 4.6 ± 0.49 | 0.33 ± 0.01 | 15.3 ± 3.2 |
Nodules with roots | 0.80 ± 0.19 | 36.9 ± 8.7 | |||
S4 Solid Heterogeneous |
Plantlet leafs | 119.8 ± 8.3 | 6.7 ± 0.4 | - | - |
Petiole | 0.23 ± 0.04 | 115.4 ± 2.7 | |||
Main stems | 0.29 ± 0.09 | 19.1 ± 6.4 | |||
Plantlets roots | 0.66 ± 0.06 | 44.2 ± 4.0 | |||
S5 Solid Heterogeneous |
Deformed Stems | 102 ± 15.2 | 7.7 ± 0.76 | 0.20 ± 0.01 | 15.7 ± 0.7 |
Suspension culture S1 was cultivated for 10 days, S2 for 25 days and the callus culture S3, S4, S5 for 30 days. The data (mean ± SE) was obtained from six independent experiments with 10 replicates each.