. 2019 Feb 20;20(4):913. doi: 10.3390/ijms20040913

Table 1.

Primers used to transfer TB40/E fragment ‘A’ to AD169.

Primers	Sequences *	Purpose
A-MssI-Fwd	CCCCGTAAACGATATAAGCGCTATCGCCAGATATCGCGTA gtttaaacGATACGCGAGCGAACGTGA	Cloning of UL112-127 of TB40/E in pBR322
A-SmiI-Rev	AAACTACGTCACCCGACACGCGGAAAAGAAAGACCGTCGC atttaaatTTCTTAGACGTCAGGTGGCAC	Cloning of UL112-127 of TB40/E in pBR322
a-Del-Fwd	TCCTCTTGTAGCAACGTGAGGACGACTACTCCGTGTGGCTCGACGGTACG TGTTGACAATTAATCATCGGCAT	Deletion of UL112-127 in AD169 and replacement with zeo^R
a-Del-Rev	GTGTGTCGCAAATATCGCAGTTTCGATATAGGTGACAGACGATATGAGGC TCAGTCCTGCTCCTCGGCCA	Deletion of UL112-127 in AD169 and replacement with zeo^R
A-Ins-Fwd	CCATTTACCGTAAGTTATGTAACGCGGAACTCCATATATGGGCTATGAACTAATGACCCC TAGGGATAACAGGGTAATCGATTT	Insertion of kan^R/I-SceI cassette into UL112-127 of TB40/E
A-Ins-Rev	GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCC GCCAGTGTTACAACCAATTAACC	Insertion of kan^R/I-SceI cassette into UL112-127 of TB40/E

* Homologous sequences are in italics, restriction sites in lower case, and PCR binding sequences in bold.