Figure 2.

Dot1L knockout promotes cell migration of ovarian cancer cells. (A) OVCAR3 and OVCAR4 cells were infected with the Cas9 and sgRNA-expressing or control (Cas9 only) lentivirus, after selected in puromycin, the cells were subjected to trans-well migration assays. Representative images of cell migration were shown. (B and C) Same as (A) but quantified for crystal violet staining of OVCAR3 cells (B) and OVCAR4 cells (C). A total of 200 cells were examined for each of the indicated groups, error bars represent mean of three independent experiments with SD. **P < 0.01.