Figure 4.

Dot1L knockout promotes cancer stem cell like properties in ovarian cancer cells. (A) ALDH activity was measured by FACS using ALDEFLUOR^TM Kit in control or Dot1L knockout OVCAR3 and OVCAR4 cells. DEAB was used as negative control for ALDH activity, the percentages of positive cells are indicated. (B) Quantification of (A). Error bars represent mean of three independent experiments with SD. **P < 0.01. (C) The expression level of ALDH1A1 was detected by western blotting in control or Dot1L knockout OVCAR3 and OVCAR4 cells. β-actin was used as loading control here.