Figure 5.

Comparison of Treg suppressive function between Jak2^-/- and control recipients six days after transplantation. A. Comparison of splenic Treg numbers between Jak2^-/- and control recipients. B. Analysis of Tregs in the draining lymph nodes (DLN) of recipients. C. A representative result for Treg suppressive assays. Proliferation of Tresps upon anti-CD3 stimulation in the presence of accessory cells along with different proportion of Tregs was estimated by flow cytometry based on the halving of CFSE fluorescence after three days of culture. D. Results for Treg suppressive kinetics of all recipients studied for each group. Four recipients were included for each group and the studies were carried out with three replications.