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. 2019 Feb 15;11(2):964–973.

Figure 1.

Figure 1

BNP treatment counteracts the profibrogenic effect of TGFβ1 in podocytes, associated with a restored ECM catabolic activity. Differentiated immortalized murine podocytes were treated with vehicle, BNP (10-6 M, 10-7 M, 10-8 M) and/or TGFβ1 (10 ng/ml) for 48 h. A. Cell cultures treated with vehicle, BNP (10-6 M) and/or TGFβ1 (10 ng/ml) for 48 h were stained with Sirius red and visualized under light microscope. Representative microscopic images were shown. Bar =100 µm. B. Cell lysates were collected and processed for Sirius red total collagen assay. Relative abundance of total collagen is shown. *P < 0.05 versus BNP-treated cells (n=3). C. Podocytes were grown on ECM pre-labelled with [3H]proline and differentiated. Cells were then treated with vehicle, BNP (10-6 M, 10-7 M, 10-8 M) and/or TGFβ1 (10 ng/ml) for 48 h followed by pulse-chase analysis of the ECM catabolic activity as specified in the Methods and expressed here as the arbitrary value relative to the vehicle treated control group. *P < 0.05 versus BNP-treated cells (n=3).