Figure 1.

GRP78 is upregulated in hypoxic rat PASMCs. A. The purity of PASMCs was identified by αSMA immunofluorescence staining (magnification, × 200). B. GRP78 protein expression in PASMCs treated with different oxygen concentrations. C. GRP78 protein expression in PASMCs treated with the indicated times of hypoxia. D. Immunofluorescence staining of GRP78 visually verified ERS changes under hypoxia and normoxia. Data are shown as the mean ± SEM; n = 3. *P < 0.05, **P < 0.01. *compared with the normoxia group or 0 h time point; 0 h was considered to be a control point, which was compared with all of the other time points.