Figure 4.

PASMC proliferation and migration were inhibited by 4u8c. A. CCK8 tests were performed to measure cell viability after 48 h of treatment, and OD values were analyzed. B, C. Expression of proteins related to proliferation and migration was evaluated. D, E. CFSE dilution analysis of PASMCs after CFSE labeling. F. Crystal violet staining of PASMCs in a transwell assay (original × 100, scale bars: 100 µm). G. The number of migrated cells was counted and analyzed. Data are shown as the mean ± SEM; n = 3. *P < 0.05, **P < 0.01 compared with the normoxia group; ^#P < 0.05, ^##P < 0.01 compared with the hypoxia group.