Figure 7.

Quercetin and 4u8c have an additive effect on the proliferation and migration of hypoxic PASMCs. A, B. CFSE dilution analysis of PASMCs was performed by pretreating with 4u8c for 24 h, and quercetin was added for another 24 h. C, D. Protein expression related to proliferation was evaluated. E, F. Transwell assays were performed by adding 1 × 10⁴ cells per transwell chamber, pretreating cells with different doses of 4u8c for 12 h and adding quercetin (60 mM) for another 12 h in the lower chamber. The number of migrated cells was counted and analyzed. Qu, quercetin. Data are expressed as the mean ± SEM; n = 4. *P < 0.05 compared with the normoxia group, ^#P < 0.05, ^##P < 0.01 compared with hypoxia-treated PASMCs, ^&P < 0.05, ^&&P < 0.01 compared with quercetin-treated PASMCs.