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. 2019 Feb 15;11(2):733–743.

Figure 6.

Figure 6

CCR4 knockdown and ophiopogonin B (OP-B) affected the levels of EMT-related proteins in CCL2 or CCL5 treated HPAEpic cells. (A, B) HPAEpic cells were transduced with CCR4 shRNAs (sh#1, sh#2 and sh#3) or control shRNA (shCon). The mRNA and protein expression of CCR4 was assessed by real-time PCR (A) and western blotting analysis (B), respectively, at 48 h post transduction. Cells without any treatment (Mock) were used as negative control. ***P<0.001 versus shCon. (C) HPAEpic cells were divided into 7 groups and pre-treated with shCon or sh#3 for 24 h, or with 4 mM OP-B for 2 h, and then exposed to 100 ng/ml CCL2 (R&D Systems, Minneapolis, MN, USA) or CCL5 (R&D Systems) or not as indicated for 24 h. The levels of E-cadherin, Vimentin, p-ERK and ERK was detected by western blotting.