Figure 1.

SXBX pill inhibits high fat diet mediated dedifferentiation of VSMCs in vivo. A. Immunofluorescence double staining showing the overlap of SMA (green) and OPN (red) in aortic of LDLR^-/- mice continuously received normal chow diet (control), high fat diet (HFD), HFD + Low SXBX pill (45 mg/kg/d) and HFD + High SXBX pill (90 mg/kg/d) for 14 weeks. Nuclei were stained by DAPI (blue). Bar = 50 μm. B. Relative mRNA levels of SMA, SM22α and OPN in the aorta of LDLR^-/- mice (n = 10). C. The expression levels of contractile protein α-SMA, SM22α and synthetic proteins OPN in the aortic of mice from above four groups (n = 10). *P<0.05 vs. control group; #P<0.05 vs. Hcy group.