Figure 2.

Transfection of siPDCD5-1 inhibits PDCD5 expression at protein level. Western blotting assessment on the effects of siPDCD5 on endogenous PDCD5 protein expression. Saos-2 and U2OS were transfected with non-silencing siRNA or siPDCD5s. At 24 hours post transfection, endogenous PDCD5 protein expression was measured by Western blotting. Significant decreased expression of endogenous PDCD5 was observed in siPDCD5-1 transfected cells, whereas in non-silencing siRNA transfected cells, endogenous PDCD5 shows no decrease. GAPDH serves as the internal control.