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Metformin directs M2 macrophages polarization involved in mTOR signaling pathway. mTOR activator MHY1485 can disturb the therapy effect of metformin. A. Representative images of immunofluorescence analysis of macrophage phenotype in vitro. Scale bar 50 μm. B, C. Representative images and quantitation of the western blot showing the protein expression of p-mTOR, mTOR. The original images are available in Figure S4A. Data are presented as the mean ± SEM, *P<0.05 vs. Met group.
