Figure 2.

Effect of BBR on hypoxia/HG-induced apoptosis in NRK-52E cells. A. Western blots were performed with the antibodies indicated. Relative expression of apoptosis proteins was calculated and normalized to the loading control. B. The apoptosis was determined by flow cytometry, followed by Annexin V-PI double staining. The data were mean ± SEM (n = 6). (**P < 0.001, vs. control, ##P < 0.001, vs. hypoxia /HG). C-H. Corresponding protein levels were assessed using densitometry and expressed in relative intensities. All results were obtained from three independent experiments. (**P < 0.001, vs. control, ##P < 0.001, vs. hypoxia/HG, #P < 0.05, vs. hypoxia/HG).