KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-GFP | Abcam | Cat# ab6556; RRID:AB_305564 |
| Guinea pig anti-alpha 1A | Synaptic systems | Cat# 152205; RRID:AB_2619842 |
| 6 nm colloidal Gold-AffiniPure Donkey anti rabbit IgG | Jackson ImmunoResearch | Cat# 711005152; RRID:AB_2340585 |
| 12 nm colloidal Gold-AffiniPure Donkey anti guinea pig IgG | Jackson ImmunoResearch | Cat# 706205148; RRID:AB_2340465 |
| Nanogold IgG goat anti rabbit IgG (H+L) Antibody | Nanoprobes | Cat# 2003; RRID:AB_2687591 |
| Bacterial and Virus Strains | ||
| HdAd 28E4 pun CaV2.1 α1 syn mEGFP | Young Laboratory, University of Iowa | N/A |
| HdAd 28E4 pun CaV2.1 α1 syn EGFP | Young Laboratory, University of Iowa | N/A |
| HdAd 28E4 pun CaV2.2 α1 syn EGFP | Young Laboratory, University of Iowa | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Kynurenic acid | Tocris Bioscience | Cat# 0223 |
| Lidocaine N-ethyl bromide (QX-314) | Sigma Aldrich | Cat# L5783 |
| D-AP5 | Tocris Bioscience | Cat# 0106 |
| (−)-Bicuculline methochloride | Tocris Bioscience | Cat# 0131 |
| Strychnine hydrochloride | Tocris Bioscience | Cat# 2785 |
| 4-Aminopyridine | Tocris Bioscience | Cat# 0940 |
| Tetraethylammonium chloride | Sigma Aldrich | Cat# T-2265 |
| Tetrodotoxin | Alomone labs | Cat# T-550 |
| ω-Agatoxin AgaIVA | Alomone labs | Cat# STA-500 |
| ω-Conotoxin GVIA | Alomone labs | Cat# C-300 |
| Cadmium chloride hemi(pentahydrate) | Sigma Aldrich | Cat# C3141 |
| Lucifer Yellow | Sigma Aldrich | Cat# L0259 |
| Experimental Models: Organisms/Strains | ||
| Mice: C57BL/6J | Jackson Laboratory | Cat# JAX:000664; RRID:IMSR_JAX:000664 |
| Oligonucleotides | ||
| Ad5 E1a (RCA) probe: NED-5’-AGCACCCCGGGC ACGGTTG-3’-MGBNFQ | This study | N/A |
| Ad5 E1a (RCA) fwd: 5’-GGGTGAGGAGTTTGTGT TAGATTATG-3’ | This study | N/A |
| Ad5 E1a (RCA) rev: 5’-TCCTCCGGTGATAATGAC AAGA-3’ | This study | N/A |
| E2B Iva2 (HV) probe: FAM-5’-TGTCTTTCAGTAGC AAGCT-3’-TAMRA | This study | N/A |
| E2B Iva2 (HV) fwd: 5’-TGGGCGTGGTGCCTAAAA-3’ | This study | N/A |
| E2B Iva2 (HV) rev: 5’-GCCTGCCCCTGGCAAT-3’ | This study | N/A |
| Genomic stuffer DNA (HdAd) probe: VIC-5’- AGC CTCTCTCATCTCACAGT -3’-MGBNFQ | This study | N/A |
| Genomic stuffer DNA (HdAd) fwd: 5’-CCCCGCTA CCCCAATCC-3’ | This study | N/A |
| Genomic stuffer DNA (HdAd) rev: 5’-TTAGCTTTTT TGGGTGATTTTTCC-3’ | This study | N/A |
| CaV2.1 α1 subunit cDNA (Mus musculus) | NCBI | NP_031604.3 |
| CaV2.2 α1 subunit cDNA (Mus musculus) | NCBI | NP_001035993.1 |
| Software and Algorithms | ||
| Matlab | The Mathworks | RRID:SCR_001622; v9.3, v9.5 |
| Patchmaster | HEKA; Harvard Bioscience | RRID:SCR_000034; v2×90.2 |
| Igor Pro | Wavemetrics | RRID:SCR_000325; v6.37 |
| Prism | Graphpad software | RRID:SCR_002798; v7.04 |
| Fiji | https://fiji.sc/ | RRID:SCR_002285 |
| Fitmaster | HEKA; Harvard Bioscience | RRID:SCR_016233; v2×90.2 |
| Patcher’s Power Tools | Max Planck Institute for Biophysical Chemistry; Gottingen; Germany | RRID:SCR_001950; v2.19 |
| Microscopy Image Browser | Electron Microscopy Unit; Institute of Biotechnology; University of Helsinki; Finland | RRID:SCR_016560; v2.4 |
| StereoDrive | Neurostar | N/A; v3.1.5 |
| Live Acquisition | Thermo Fisher Scientific | N/A; v2.1.0.10 |
| QCapture | QImaging | RRID:SCR_014432; v7.0 |
| NpRf model (Matlab function) | This study | https://doi.org/10.17632/4ycrjtpfc6.1 |