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. Author manuscript; available in PMC: 2019 Mar 12.
Published in final edited form as: Sci Signal. 2018 Sep 11;11(547):eaan1210. doi: 10.1126/scisignal.aan1210

Fig. 6. PI4P depletion at the PM blocks total IP production.

Fig. 6.

(A to C) Analysis of total IP production in vehicle- or ET-1–treated MEFs (left) and in vehicle- or NT-treated PANC-1 cells (right) after global PI4P depletion with PAO (A), after PM PI4P depletion with A1 (B), and after Golgi PI4P depletion with PIK93. The indicated cells were serum-starved and labeled with [3H]inositol for 24 hours and then were pretreated for 15 min with 10 μM PAO, 100 nM A1, or 300 nM PIK93 before being treated for 30 min with vehicle, 100 nM ET-1, or 100 nM NT in the presence of LiCl. The results are expressed in counts per minute (left) or fold over unstimulated cells (right). Data are means ± SD and were analyzed by one-way ANOVA with Tukey’s post-test. **P < 0.01 and ****P < 0.0001.