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In vitro kinase inhibition by HSN431. The dose-response curves obtained by incubating increasing concentrations of HSN431 with kinases in the (a) PI3K/AKT/mTOR pathway and (b) MAPK pathway. ATP concentration was 100 μM. Refer to Table S1 for concentrations of kinases and corresponding substrates. Each data point represents the mean ± SD of duplicate measurements.
