Fig. 8.

The effect of HSN431 on cell cycle distribution. DMSO (vehicle) or HSN431 at the indicated concentrations was incubated with (a) MV4–11, (b) MOLM-14, (c) MOLM-14 (ITD, D835Y) and (d) MOLM-14 (ITD, F691 L) cells for 24 h and 48 h. Cells were harvested by centrifugation, washed, fixed in 70% ethanol and stained with propidium iodide (PI) before being analyzed by flow cytometry. For each stacked bar, the distribution of cells in the G1 phase (blue), S phase (red), G2 phase (green) and sub-G1 (magenta) have been indicated by their percentages. Samples were analyzed in triplicates on a FACSCanto II flow cytometer (BD Bioscience, San Jose, CA, USA) and data were analyzed with FlowJo software (Flowjo, LLC, Ashland, OR, USA). Data represent the mean ± SD of triplicate measurements. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)