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. 2019 Jan 25;40:163–175. doi: 10.1016/j.ebiom.2019.01.030

Fig. 2.

Fig. 2

SLC2A4RG controls glioma cell survival in vitro. (a) Western blot was used to examine the expression efficiency of SLC2A4RG in U87 and U251 cells with SLC2A4RG overexpression or knockdown. β-Actin serves as the loading control. (b) The growth curves of U87 and U251 cells with overexpression or depletion of SLC2A4RG described from day 1 to day 5. (c) The Growth of U251 cells with SLC2A4RG overexpression or depletion observed by colony formation assay. (d) The Edu/DAPI rate was calculated in primary human glioma G1 cells transfected with vector or SLC2A4RG. (e) Flow cytometry was detecting the cell cycle changes of U87, U251, and G1 cells with overexpressed or depleted SLC2A4RG. (f) The effects of SLC2A4RG overexpression or depletion on the expression of cell cycle-related proteins examined by Western blot. β-Actin serves as the loading control. (g) Flow cytometry with Annexin V and 7-AAD staining determining the changes of apoptosis in U87 and U251 cells under the condition of SLC2A4RG overexpression. Results analyzed by t-test presented as mean ± SEM. *, P < 0.05; **, P < 0.01; ***, P < 0.001.