Figure 1.

Gene expression of HES1, HEY1, and HEY2 1 and 24 h after a single loading session in left-loaded and right-nonloaded osteocyte-enriched tibiae of 10-week-old female C57BL/6 mice. Fold changes in gene expression of loaded limbs (n = 6) normalized to control limbs are shown (a) 1 h and (b) 24 h after loading. qPCR data were obtained from technical duplicates. Results are shown as mean ± SD; fold change was calculated with the ΔΔCt method and normalized to basal expression (nonloaded right limb, dashed line). GAPDH served as the housekeeping gene. Two-tailed paired t-test was used for statistical analysis (^∗P < 0.05 and ^∗∗∗P < 0.001).