Fig. 1.

LAMP2a is upregulated in TAMs and correlates with overall survive in breast cancer.

(a) Representative immunofluorescence images of tumor sections from MMTV-PyMT mice, labeled by F4/80 (red), LAMP2a (green) and DAPI (blue). Scale bars are marked in individual images.

(b) LAMP2a expression in tumor cells (CD45⁻), TIMs (F4/80⁺) and mTAMs was detected by flow cytometry in PyMT mice. n = 5/group.

(c) LAMP2a expression in tumor cells (CD45⁻) and TAMs (F4/80⁺) was detected in 4 T1 and CT26 inoculation mouse models by flow cytometry. n = 5/group.

(d) and (e) LAMP2a expression in tissue macrophages (d) and tumor stroma cells (e) was detected by flow cytometry in PyMT mice. n = 4–6/group.

(f) Representative immunofluorescence images of tumor tissues or exfoliated cells from clinical samples. Sections were labeled with CD68 (green), LAMP2a (red), DAPI (blue) or CD163 (red), LAMP2a (green), DAPI (blue) as marked in images. Scale bar, 20 μm.

(g) Survival curves of breast cancer patients grouped by LAMP2a expression either in tumor cells (upper panel) or stromal cells (bottom panel), analyzed by Log-rank (Mantel-Cox) test. Immunohistochemistry images with labeled LAMP2a were representative regions of LAMP2a expression in tumor cells (upper panel) and stromal cells (bottom panel).

Data are represented as mean ± SD, with Student's t-tests unless noted. *p < 0·05, **p < 0·01, ***p < 0·001. Data represent 2–3 independent experiments. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)