Fig. 5.

Tumor immuno-environment is restored by LAMP2a inhibition.

(a) and (b) Cell population of mTAMs, MDSCs (a) and IMCs (inflammatory monocytes) (b) from PyMT mice. Flow cytometric graphs were representative results, red and blue gates in MDSC graphs indicate M-MDSC and PMN-MDSC respectively. n = 4–6/group.

(c) and (d) Cell population of CD4⁺ IFN-γ⁺, CD4⁺ IL-4⁺(c) and CD8⁺ IFN-γ⁺, CD8⁺ PD-1⁺ T cells (d) from PyMT mice. Flow cytometric graphs were representative results. n = 4–6/group.

(e) TUNEL staining of tumor tissue sections from PyMT mice which received NS, sh-NC or sh-L2a transfection. Scale bars are marked in individual images.

Data are represented as mean ± SD, with one-way ANOVA tests. *p < 0·05, **p < 0·01, ***p < 0·001, ns, no significance. Data represent 2–3 independent experiments. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)