Fig. 2.

Recombinant expression of NT5C1A in human and murine pancreatic cancer cell lines. A) and B) Representative images of two biological replicates of immunocytochemistry staining for NT5C1A and HA-tag. Robust staining is shown for HA-tag (A, lower panel) and NT5C1A (B, lower panel) in transfected KPC-BL6 (=KPC2) cells. No staining of HA-tag (A, upper panel) and low endogenous NT5C1A expression (B, upper panel) were detected in vector control cells (n = 2), scale bars 50 μm. C) Quantitative RT-PCR analysis confirmed overexpression of NT5C1A in transfected cells. Diagram indicates mean ± SEM of three biological replicates. Murine muscle sample was used as positive control and values were normalized to the housekeeping gene β-actin. D) Western blot analysis confirmed NT5C1A protein expression in transfected tumor cells with hardly any expression in vector control cells. Robust expression of HA-tag is shown in all +NT5C1A cell lines. Representative image of three independent experiments is shown. Murine muscle lysate was used as positive control for NT5C1A expression.