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. Author manuscript; available in PMC: 2019 Mar 12.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Sep 25;440(3):431–436. doi: 10.1016/j.bbrc.2013.09.090

Fig. 3.

Fig. 3

(A) Fem1b promotes the ubiquitylation of Gli1. 293T cells were transfected with the indicated constructs before co-immunoprecipitation with a Myc antibody followed by Western blot. Inputs represent 7% of the WCL. (B) Gli1 protein is stabilized by the proteasome inhibitor MG132. 293T cells were transfected with the indicated constructs for 9 h and then treated or untreated with 10 μM of the proteasome inhibitor MG132 for 3.5 h before Western blotting.