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. 2018 Nov 2;21(2):143–149. doi: 10.4103/aja.aja_78_18

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Copyright: © The Author(s)(2018)

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(a) Immunohistochemical staining of penile cavernous tissues for α-SMA, calponin-1 and OPN in control and hyperlipidemia groups (magnification ×100, scale bars = 100 μm; ×200, scale bars = 50 μm). (b) Western blot analysis showed phenotypic transition in SMCs. Lanes 1–3: control; lanes 4–6: hyperlipidemia. Reduced expression of α-SMA and calponin-1 was demonstrated in hyperlipidemia samples. On the contrary, OPN and p-MLC₂₀ were overexpressed in hyperlipidemia compared with control samples (n = 6 per group). ^#P < 0.01, hyperlipidemia group versus control group. α-SMA: smooth muscle α-actin; OPN: osteopontin; SMC: smooth muscle cell; p-MLC₂₀: phospho-myosin light chain₂₀.