Figure 2.

The stem cells derived from AIMP3 TG mice exhibited compromised stem cell properties. (a) Oil Red O staining with primary adipose‐derived MSCs (AD‐MSCs) from 3‐ and 10‐month‐old AIMP3 TGs (3 M and 10 M each) revealed compromised adipogenic potential in even 3 M AD‐MSCs compared to the littermate controls, and the differentiation deficits became more evident in the AD‐MSCs from 10 M AIMP3 mice. The scale bars represent 50 μm. WT n = 3 and AIMP3 TG n = 4 mice of each age. (b) Four key transcription factors for adipogenesis were significantly suppressed in AD‐MSCs from 3 M AIMP3 TG mice at p6. **p ≤ 0.01; ***p ≤ 0.001. Ap2: adipocyte fatty acid‐binding protein; CEBPα: CCAAT/enhancer‐binding protein alpha; CIDEC: cell death‐inducing DFFA‐like effector C; PPARγ: peroxisome proliferator‐activated receptors gamma. The 36B4 gene, an acidic ribosomal phosphoprotein P0 (RPLP0), was used for normalization