Figure 2.

Vascular aging of renal arterioles was reduced in RAGE^−/− mice. (a) Representative PAS staining of paraffin‐embedded kidney sections of WT (left panels) and RAGE^−/− mice (right panels) fed a control (upper panels) or CML‐enriched diet (lower panels) revealed the presence of hyalinosis (arrowheads), tubules undergoing tubular atrophy (arrows) and glomerulosclerosis (asterisks) (x400). (b) Kidney arteriolar hyalinosis determined by PAS staining in WT and RAGE^−/− mice and (c) alteration in endothelium‐dependant relaxation in 11‐month‐old and 20‐month‐old WT and RAGE^−/− mice fed a control diet. Aortic relaxation is expressed as mean ±SEM (n = 5–6 mice in duplicate). **p < 0.01, ***p < 0.001, unpaired t test on area under the curve