Figure 6.

Inflammation and oxidation markers were decreased in RAGE^−/− mice. Inflammation markers (a) Il‐6, (b) Tnfα and (c) Vcam‐1 and oxidation markers (d) Sod2, (e) Cat and (f) HmoxmRNA expression were measured in renal tissue from 3‐ or 20‐month‐old WT and RAGE^−/− mice (mean ±SEM, n = 3 for 3‐month‐old mice and n = 10 for 20‐month‐old mice). Representative western blot and quantification of protein levels of (g) SIRT1 (mean ±SEM, n = 10), (h) pS6RP (Ser235/236)/S6RP and (i) pAKT (Ser473)/AKT in kidney extracts of 22‐ to 26‐month‐old mice (mean ±SEM, n = 4). *p < 0.05, **p < 0.01, Mann–Whitney test or Kruskal–Wallis test for multiple comparisons