Figure 1.

Vector construction and short‐term hypothalamic gene delivery. (a) AAV vector constructs used in study. CBA: cytomegalovirus enhancer plus chicken ß‐actin promoter; polyA: bovine growth hormone polyadenosine tail; WPRE: woodchuck posttranscriptional regulatory element. (b) Body weight. (c) Glucose tolerance test performed 57 days post‐AAV injection. (d) Area under the curve (AUC) of (c). (e) Relative fat mass at sacrifice 63 days post‐AAV injection. BAT: brown adipose tissue; gWAT: gonadal white adipose tissue; iWAT: inguinal white adipose tissue; rWAT: retroperitoneal white adipose tissue. (f) Serum biomarkers at sacrifice. (g) YFP fluorescence. Scale bar, 100 µm. ARC: arcuate nucleus; VMH: ventromedial hypothalamus; 3V: third ventricle. (h) BDNF protein level in hypothalamic dissections. Error bars represent mean ± SEM. n = 10 per group (b–e); n = 7 per group (h). *p < 0.05. **p < 0.01. ***p < 0.001. ^# p < 0.06