Figure 6.

Deacetylation‐independent modulation of p53 accumulation by Sirt1. (a) Immunofluorescence of p53 and the quantitative data showing the effect of H363Y mutant (HY) of Sirt1 on ActD‐induced p53 accumulation (localized primarily in the nuclei). (b) Immunofluorescence of p53 showing the effect of wild‐type Sirt1 on ActD‐induced p53 accumulation in EX‐527 pretreated cells. (c) Western blot and quantitative densitometry data showing the time course of ActD‐induced p53 accumulation. (d) Western blots showing the effect of Sirt1‐H363Y on p53 protein level in resting and ActD‐treated cells. (e) Effects of wild‐type Sirt1 (WT) and Sirt1‐H363Y (HY) on ActD‐induced accumulation of ectopically expressed eGFP‐p53‐8KR (Western blot was performed using an anti‐eGFP antibody). All experiments were performed using HeLa cells. *p < 0.05, one‐way ANOVA, n = 3–5