Figure 3.

Effects of ACA on fat depends on specific fat type. Effects of ACA on amount of fat relative to body weight in 12‐month‐old HET3 mice at UM. Numbers of male ACA and controls are 9 each, while 8 females received ACA and 6 were controls. In subscapular fat, ACA effects are sex‐specific: females, p = 0.71; males, p < 0.001. In mesenteric fat, ACA results in a strong reduction in both sexes: p < 0.001. In gonadal fat, ACA effects are similar in both sexes, but weak: p = 0.074. In Inguinal fat, ACA has no effect. p values, from analysis of covariance, reflect differences in the intercept term, which measures whether ACA alters fat pad weight after adjustment for body weight. HET3 mice were fed diet with 1,000 ppm ACA starting at 4 months; controls were fed the base diet. Mice were fasted for 18 hr prior to dissection. This was part of a larger study in which mice had a sham‐operation procedure at age 3 months. They were anesthetized, gonads were exteriorized through an incision and then returned to the abdominal cavity, and the wound closed