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. 2019 Jan 9;40:488–503. doi: 10.1016/j.ebiom.2018.12.056

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© 2019 The Authors. Published by Elsevier B.V.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 5 — LRH1 protection of the liver from toxicant-induced injury.

(A) LRH1 protection of the liver from APAP-induced injury. H&E staining (upper left). At four days after a hydrodynamic injection of the plasmid encoding LRH1 or mock vector (pcDNA3.1), mice were fasted overnight and subjected to a single dose of APAP (300 mg/kg), and the liver tissues were obtained six h afterward. TUNEL staining (upper right). The scale bars represent 100 μm. Serum ALT and AST activities (lower left). Liver weight per body weight ratio (middle). Correlation between serum ALT activities and LRH1 transcript levels in the liver (lower right).

(B) LRH1 protection of the liver from CCl₄-induced injury. H&E staining (upper left). Mice were subjected to a single dose of CCl₄ (0.6 mL/kg) four days after a hydrodynamic injection of the plasmid encoding LRH1 or mock vector (pcDNA3.1), and the liver tissues were obtained two days afterward. TUNEL staining (upper right). TUNEL-stained tissues were separated to non-tissue, normal and apoptotic areas by blue, green and red colors, respectively. Insets showed true-colour images. The scale bars represent 100 μm. Serum ALT and AST activities (lower left). Correlation between serum ALT activities and LRH1 (Nr5a2) mRNA levels in the liver (lower right).

(C) Immunoblottings for apoptosis or liver regeneration markers (left). Values were obtained using scanning densitometry (right).

Data information: For A, data represent the means ± SEM (Mock+Veh, n = 7; Mock+APAP, n = 8; LRH1 + APAP, n = 13; and non-injected control (Con), n = 5, significantly different as compared to vehicle control, **P < .01; or APAP-treated control, #P < .05; ##P < .01). For B, data represent the means ± SEM (Mock+Veh, n = 6; Mock+CCl₄, n = 14; LRH1 + CCl₄, n = 4; and non-injected control (Con), n = 4, significantly different as compared to vehicle control, **P < .01; or CCl₄-treated control: #P < .05; ##P < .01). For C, data represent the means ± SEM (n = 4 each, significantly different as compared to vehicle control, *P < .05; **P < .01; or CCl₄-treated control: #P < .05; ##P < .01). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)