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. 2019 Jan 25;40:184–197. doi: 10.1016/j.ebiom.2019.01.036

Fig. 3.

Fig. 3

SLC25A11 knockdown induced cell death following cell growth arrest through OxPhos reduction in melanoma and NSCLC cells. (a) Clonogenic assay was performed in NSCLC, and melanoma cells were treated with control or SLC25A11 siRNA (40 nM) for 2 weeks. (b) Cell death was measured using an Annexin V staining kit in cells treated with SLC25A11 siRNA (40 nM) for the indicated times. (c) Cell proliferation assay using SRB assay and ATP assay were performed with IMR90 cells by transfection of SLC25A11 siRNA (40 nM) or control siRNA for 48 h. (d) The mitochondrial membrane potential of H1975, UACC62, and IMR90 cells treated with SLC25A11 siRNA for 48 h was analyzed by live cell imaging using ZEN software (scale bar, 50 μm, 10 μm). (e) The oxygen consumption rate was analyzed using the Seahorse XFe analyzer in A549 and UACC62 cells treated with control or SLC25A11 siRNA (40 nM) for 24 h and then normalized by SRB assay. (Data were presented as mean ± SD. ***p < .001, **p < .01, *p < .05).