Fig. 1.

CLLV-1 attenuates superoxide anion generation, ROS formation, and p47^phox phosphorylation in fMLF-activated human neutrophils. (a) The chemical structure of CLLV-1. (B–C) Human neutrophils were preincubated with DMSO or CLLV-1 (0.03–3 μM) and then activated with or without fMLF (0.1 μM)/CB (1 μg/mL). (b) Superoxide anion generation was detected using cytochrome c reduction by a spectrophotometer at 550 nm. (c) The intracellular ROS was monitored by flow cytometry, using cell-permeable DHR123. (d) Phosphorylation of p47^phox was analyzed by immunoblotting, using antibodies against the phosphorylated (S304) and total p47^phox. All data are expressed as mean values ± SEM (n = 3); *p < .05, **p < .01, and ***p < .001 compared with the DMSO + fMLF group (Student's t-test).