Fig. 7.

CLLV-1 decreases the AMS-labeled reduced form of AKT and increases the AKT-PP2A interaction in fMLF-activated dHL-60 cells. dHL-60 cells were incubated with CLLV-1 (0.3–3 μM) for 5 min before stimulation with or without fMLF (0.1 μM)/CB (1 μg/mL). (a) The cell lysates were incubated with the thiol-alkylating agent AMS for 12 h on ice and analyzed by Western blotting under reducing (total lysate) or non-reducing conditions (AMS-labeled AKT). The AMS-labeled reduced form of AKT (Red) had a higher molecular weight than oxidized AKT (Oxi). (b) The cell lysates were immunoprecipitated with control (Ctrl) or AKT IgG. The precipitated substances were used for Western blotting of AKT and PP2A. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)