Fig. 8.

CLLV-1 attenuates LPS-induced ALI in mice. C57BL/6 mice were intraperitoneally injected with CLLV-1 (10 mg/kg), MK-2206 (10 mg/kg), or an equal volume of DMSO for 1 h, and subsequently instilled 2 mg/kg LPS from E. coli 0111:B4 or 0.9% saline via tracheostomy. (a-b) Six hours later, lungs were collected and assayed for MPO activity (a) and protein levels (b). (c) Histological examination of lungs. The lung sections were stained with hematoxylin and eosin (H&E), anti-Ly6G antibodies, or anti-pAKT (S473) antibodies by IHC. All data are expressed as mean values ± SEM (n = 6); **p < .01 and ***p < .001 compared with the LPS + DMSO group; ^###p < .001 compared with the DMSO control (Student's t-test).