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Exon spanning primers were designed from the rat sequences of Scnn1a, Scnn1b, Scnn1g, Aqp2, and 18s.
| 18S F | CGGCTACCACATCCAAGGAA |
| 18S Rev | CCTGTATTGTTATTTTTCGTCACTACCT |
| Scnn1a F | CCCTGCAACCAGGCGAATTA |
| Scnn1a Rev | TCCTGACCATGCACCATCAC |
| Scnn1b F | GAGCTGCCTTCTTGGGTTCT |
| Scnn1b Rev | CCACACGATATTGTTGGCCG |
| Scnn1g F | TCACGCTTTTCCACCATCCA |
| Scnn1g Rev | GATGACTTGCAGCCCGTACT |
| Aqp2 F | GCCACCTCCTTGGGATCTATT |
| Aqp2 Rev | AAGACCCAGTGATCATCAAACTTG |
