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. 2019 Feb 14;13:39. doi: 10.3389/fncel.2019.00039

Figure 1.

Figure 1

Comparison of densely and sparsely expressed GEVIs. (A) The microscope set up for wide-field imaging. We used a standard epifluorescence microscope configuration to image patched cells expressing GEVIs in sparsely and densely expressing brain slices. (B) A confocal image of a brain slice densely expressing chimeric VSFP-butterfly. Assignment of fluorescence to individual neurons is impossible due to expression in overlapping membranes. (C) A wide-field image of a brain slice with the same expression strategy. The problem of assignment is compounded by the lack of optical sectioning, making even cell bodies difficult to discern. (D) A confocal image of a brain slice sparsely expressing VSFP butterfly via destabilized-Cre modulated expression. Processes and soma from individual GEVI-expressing cells can be clearly resolved. (E) Wide-field image of a brain slice with the same expression strategy. Contrast is decreased due to lack of optical sectioning, but single cells and processes can still be resolved. Scale bar 40 μm.